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axiovision colocalization module  (Carl Zeiss)


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    Carl Zeiss axiovision colocalization module
    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the <t>colocalization.</t> B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
    Axiovision Colocalization Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/measure+colocalization+module/AxioVision+4+Module+Interactive+Measurement/pmc03315488-284-8-11
    Average 96 stars, based on 1 article reviews
    axiovision colocalization module - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice"

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1002575

    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Techniques Used: Injection, Immunohistofluorescence, Expressing, Infection

    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Techniques Used: Injection, Immunohistofluorescence, Immunofluorescence, Expressing

    Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Techniques Used: Immunohistofluorescence, Expressing, Infection, Injection

    Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Techniques Used: Injection, Immunohistofluorescence, Expressing, Infection



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    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the <t>colocalization.</t> B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
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    Image Search Results


    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Injection, Immunohistofluorescence, Expressing, Infection

    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Injection, Immunohistofluorescence, Immunofluorescence, Expressing

    Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Immunohistofluorescence, Expressing, Infection, Injection

    Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Injection, Immunohistofluorescence, Expressing, Infection